《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (3): 236-238.

• 论文 • 上一篇    下一篇

舒郁宁心汤对抑郁大鼠海马Bcl-2 和Bax 表达的影响

黄泉智1，许成勇1，孙志高1，杨阳2，胡亚卓3，陈利平1，王发渭1   

  1. 1.解放军总医院中医科，北京市 100853；2.总参信息化部后勤部卫生办，北京市 100840；3.解放军总医院老年医学研究所，北京市 100853。
  • 收稿日期:2011-10-20 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25

Effect of Shuyuningxin Decoction on the Expression of Bcl-2 and Bax in Hippocampus of Chronic Stress Depression Rats

HUANG Quan-zhi, XU Cheng-yong, SUN Zhi-gao, et al.   

  1. Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2011-10-20 Revised:1900-01-01 Published:2012-03-25 Online:2012-03-25

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摘要： 目的了解舒郁宁心中药对慢性应激抑郁模型大鼠海马区凋亡调控蛋白Bcl-2 和Bax 表达的影响。方法60 只SD大鼠随机分为对照组，模型组，高、中、低剂量组和盐酸氟西汀组。复制孤养加慢性轻度不可预见性应激抑郁模型，造模成功后进行药物干预21 d。采用免疫组化方法观察大鼠海马区Bcl-2 和Bax 表达。结果与对照组比较，模型组大鼠Bax 表达升高、Bcl-2 表达降低(P<0.05)；与模型组相比，中剂量组、高剂量组、盐酸氟西汀组大鼠海马区Bax 表达降低、Bcl-2 表达升高(P<0.05)。结论舒郁宁心汤可能通过调控海马组织Bcl-2 和Bax 的表达，对抗海马神经元凋亡，发挥抗抑郁作用。

关键词: 舒郁宁心汤, 抑郁, Bcl-2, Bax

Abstract: Objective To investigate the effect of Shuyuningxin decoction on the expression of Bcl-2 and Bax in hippocampus of chronicstress depression rats. Methods 60 SD rats were randomly divided into control group, model group, fluoxetine group, high dose ofShuyuningxin decoction group, middle dose of Shuyuningxin decoction group and low dose of Shuyuningxin decoction group. All thegroups were given the chronic unpredicted mild stress for 21 d to induce depression models except the control group. Then they were administratedtheir drugs for 21 d. The level of Bcl-2 and Bax in hippocampus were investigated with immunohistochemistry technique. ResultsCompared with the control group, the level of Bax increased and Bcl-2 decreased in the model group (P<0.05). Compared with modelgroup, the level of Bax decreased and Bcl-2 increased in the middle dose of Shuyuningxin decoction group, high dose of Shuyuningxin decoctiongroup and the fluoxetine group (P<0.05). Conclusion The anti-depression effect of Shuyuningxin decoction may be associated withthe expression of Bax and Bcl-2.

Key words: Shuyuningxin decoction, depression, Bcl-2, Bax