《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (3): 222-225.

• 论文 • 上一篇    下一篇

运动负荷骤降对生长期大鼠长骨骨量和骨代谢生化指标的影响

阚世锋，余波，陈文华，张雯，祁奇   

  1. 上海交通大学附属第一人民医院康复医学科，上海市 200080。
  • 收稿日期:2011-12-27 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25

Influence of Rapid Reduction of Physical Activity on Bone Mass and Biochemical Markers of Bone Metabolism in Growing Rats

KAN Shi-feng, YU Bo, CHEN Wen-hua, et al.   

  1. Department of Rehabilitation Medicine, Shanghai First People's Hospital, Shanghai 200080,China
  • Received:2011-12-27 Revised:1900-01-01 Published:2012-03-25 Online:2012-03-25

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摘要： 目的观察运动负荷骤降对生长期大鼠长骨骨量(BMC)、骨密度(BMD)和骨代谢生化指标的影响。方法雄性3 月龄SD大鼠48 只，随机分成大强度运动组(T1)、大强度转低强度运动组(T2)、低强度运动组(T3)、对照组(Con)，每组12 只。前6 周，T1、T2 组进行大强度跑台运动，T3 组进行低强度跑台运动。6 周末，DEXA 测定每组部分大鼠股骨BMC、BMD，酶标仪法测定血清碱性磷酸酶(ALP)、骨钙素(BGP)和抗酒石酸性磷酸酶(TRACP5b)。降低T2 组运动负荷，与T3 组一致，其余组运动强度不变， 继续运动6 周。同样方法测定上述指标。结果与运动负荷降低前比较， 12 周末T2 组大鼠ALP、BGP 降低(P<0.05)，TRACP5b 升高(P<0.05)，股骨BMC、BMD无显著性差异(P>0.05)。结论运动负荷骤降使成长期大鼠长骨骨形成抑制，骨吸收增强。

关键词: 运动负荷, 骨量, 骨密度, 骨代谢, 大鼠

Abstract: Objective To observe the effect of rapid reduction of physical activity on bone mass (BMC), bone mineral density (BMD)and bone metabolism in growing rats. Methods 48 male SD rats were divided into the high-intensity exercise group (T1, n=12), high tolow-intensity exercise group (T2, n=12), low-intensity exercise group (T3, n=12) and control group (Con, n=12). During the first 6 weeks,the rats in T1 and T2 group ran on treadmill with high intensity, while rats in T3 group took low-intensity treadmill exercise. 6 rats were sacrificedfrom each group at the end of 6th week. BMC, BMD of femur were measured with DEXA. Alkaline phosphatase (ALP), osteocalcin(BGP), tartrate-resistant acid phosphatase (TRACP) were detected with ELISA. The exercise intensity of T2 group was reduced rapidly to alow level as in T3 group, but remained in the other 2 groups. At the end of the second 6 weeks, the other rats were investigated again. ResultsCompared with the first measurements, ALP and BGP of the T2 group decreased significantly (P<0.05), and TRACP increased (P<0.05). There was no significant difference in BMC and BMD of femur (P>0.05). Conclusion Rapid reduction of physical activity can suppressbone formation and stimulate bone resorption of long bone among growing rats.

Key words: physical activity, bone mass, bone mineral density, bone metabolism, rats