《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (3): 219-221.

• 论文 • 上一篇    下一篇

注射硫酸软骨素酶对大鼠脊髓损伤后腓肠肌乙酰胆碱酯酶的变化及运动功能的影响

李洪鹏1，白旭东1，高杰1，巴方2，刘宁3   

  1. 1.中国医科大学基础医学院解剖教研室，辽宁沈阳市 110001；2.中国医科大学盛京医院康复科，辽宁沈阳市 110001；3.中国医科大学附属第一医院康复科，辽宁沈阳市 110001。
  • 收稿日期:2011-09-08 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25

Effects of Chondroitinase ABC Injection on Motor and Acetylcholinestarase in Gastrocnemius of Spinal Cord Injuried Adult Rats

LI Hong-peng, BAI Xu-dong, Gao Jie, et al.   

  1. Department of Anatomy, College of Basic Medical Science, China Medical University,Shenyang 110001, Liaoning, China
  • Received:2011-09-08 Revised:1900-01-01 Published:2012-03-25 Online:2012-03-25

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摘要： 目的探讨注射硫酸软骨素酶ABC 对成年大鼠脊髓损伤后不同时期后肢运动功能的恢复及腓肠肌运动终板内乙酰胆碱酯酶的影响。方法10 周龄Wistar 雄性大鼠40 只，采用脊髓半横断法制作模型，健侧作为对照组(A组)，患侧随机分为单纯脊髓损伤组(B 组)及术后注射硫酸软骨素酶ABC 组(C 组)。术后采用BBB 评分法进行行为学观察，分别于损伤后3 d、7 d、14 d 和28d 各选取5 只大鼠，酶化学染色法检测腓肠肌中乙酰胆碱酯酶(AChE)的表达。结果C 组在术后14~28 d BBB 评分高于B 组(P<0.05)；B 组和C 组与A组相比，腓肠肌AChE 活性均降低，但C 组于术后14~28 d AchE 活性高于B 组(P<0.05)。结论大鼠脊髓损伤后注射硫酸软骨素酶可以提高AChE 的活性，并可提高大鼠患肢的运动功能。

关键词: 脊髓损伤, 硫酸软骨素酶, BBB评分, 乙酰胆碱酯酶, 大鼠

Abstract: Objective To investigate the effects of chondroitinase ABC injection on motor and acetylcholinestarase (AchE) expressed inthe motor end-plate of gastrocnemius in adult rats with spinal cord injury. Methods The spinal cords in T10 of 40 male Wistar rats (10 weeksold) were exposed and the right sites were semi-transected. The left sites were considered as the control (group A), the transected sites wereas the injured group (group B) and chondroitinase ABC-treated group (group C). The rats were assessed with Basso, Beattie & Bresnahan(BBB) score for ethological test, and sacrificed 3 d, 7 d, 14 d and 28 d after operation. The AchE expressed in gastrocnemius was detectedwith zymochemistry staining. Results BBB score in group C was significant higher than that in group B 14~28 d after operation, while theAchE activity in group B and C was lower than that in group A (P<0.05), and lower in group B than in group C (P<0.05). Conclusion ChondroitinaseABC injection can enhance AchE activity and promote the locomotor recovery after spinal cord injury in adult rats.

Key words: spinal cord injury, chondroitinase, Basso, Beattie &, Bresnahan score, acetylcholinestarase, rats