《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (2): 114-117.

• 论文 • 上一篇    下一篇

缺血性脑卒中血清低分子量蛋白质的表达

王屹，于艳，涂文军，陈蕾，陈惠   

  1. 中国康复研究中心北京博爱医院检验科，北京市 100068。
  • 收稿日期:2011-11-23 修回日期:1900-01-01 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 陈惠

Expression of Low Molecular Weight Proteins in Serum of Ischemic Stroke Patients

WANG Yi, YU Yan, TU Wen-jun, et al.   

  1. Department of Clinical Laboratory, Beijing Charity Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2011-11-23 Revised:1900-01-01 Published:2012-02-25 Online:2012-02-25

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被引次数

1

摘要： 目的探讨缺血性脑卒中患者与正常人血清蛋白表达的差异性。方法选择缺血性脑卒中患者33 例(病例组)，正常对照39 例(对照组)，通过磁珠捕获血清蛋白组分，应用基质辅助激光解析电离-飞行时间质谱(MALDI-TOF MS)技术检测蛋白质谱，获得差异多肽谱。依据美国国立卫生院神经功能缺损评分(NIHSS)标准评价卒中的严重程度，依据改良整体生活功能量表(mRS)评价功能恢复。结果病例组和对照组共获得55 个差异峰(P<0.05)。分子量1000~2000 范围中共有7 个差异峰，包括4 个正向增高的峰，3 个反向差异峰，其中反向峰质核比为1864 的峰强度在病例组中水平降低显著，其降低程度与脑卒中危险因素、卒中的严重程度、功能恢复等无相关性。结论质核比为1864 的峰在缺血性脑卒中筛查中因不受其特定人群的影响可作为反向生物标记物，为缺血性脑卒中的筛查、早期诊断和干预提供依据。

关键词: 缺血性脑卒中, 基质辅助激光解析电离-飞行时间质谱, 生物标记物

Abstract: Objective To explore the differential expression of low molecular weight proteins in serum of ischemic stroke patients.Methods Serum samples obtained from ischemic stroke patients (n=33) and normal controls (n=39) were analyzed by Matrix Assisted Laser Desorption/ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS). The severity of stroke was assessed with the National Institutes of Health Stroke Scale (NIHSS) and functional outcome was assessed with modified Rankin Scale (mRS). Results 55 peaks were significantly different between ischemic stroke patients and the controls (P<0.05). There were 7 peaks between 1000~2000 Da, 4 increased peaks and 3 induced peaks in serum of ischemic stroke patients. One induced peak at 1864(m/z) had highest peak intensity in controls and induced significantly in ischemic stroke patients. While induce degree of this peak didn't correlate with the NIHSS, mRS, stroke risk factors and laboratory data. Conclusion The application of this potential biomarker is not restricted to certain subgroups of ischemic stroke patients,so it may serve as one reverse direction biomarker and provide support for early diagnosis and treatment of ischemic stroke.

Key words: ischemic stroke, Matrix Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry, biomarker