《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (4): 342-343.

• 论文 • 上一篇    下一篇

电针对全脑缺血-再灌注大鼠IL-1β的影响

霍则军1，李志刚2，郭佳1，田兆兴1   

  1. 1.北京大学第三医院中医科，北京市 100191；2.北京中医药大学针灸学院，北京市 100029。
  • 收稿日期:2010-01-15 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25

Effects of Electroacupuncture on IL-1β after Total Cerebral Ischemia-reperfusion in Rats

HUO Ze-jun, LI Zhi-gang, GUO Jia, et al.   

  1. Department of Traditional Chinese Medicine, Peking University, 3rd Hospital, Beijing 100191, China
  • Received:2010-01-15 Revised:1900-01-01 Published:2010-04-25 Online:2010-04-25

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摘要： 目的观察电针对全脑缺血-再灌注大鼠脑组织IL-1β的影响。方法30只雄性Wistar大鼠，随机分为假手术组、全脑缺血-再灌注模型组(IR组)；全脑缺血-再灌注电针组(IRA组)，每组10只。四动脉结扎法(4-VO)建立大鼠全脑缺血-再灌注模型，作为IR组。IRA组电针大鼠曲池、足三里穴，各组均于再灌注6 h后取脑，ELISA法测定脑组织IL-1β含量。结果与假手术组和IRA组比较，IR组脑组织内IL-1β显著升高(P<0-001)，而IRA组与假手术组比较无显著性差异(P>0-05)。结论电针刺激能显著降低全脑缺血-再灌注大鼠脑组织IL-1β浓度。

关键词: 缺血-再灌注, 电针, 白细胞介素-1β(IL-1β)

Abstract: ObjectiveTo observe the effects of electroacupuncture on IL-1β in brain tissue of rats after total cerebral ischemia-reperfusion.MethodsThirty male Wistar rats were randomly divided into the sham operation group, model group and acupuncture group, in each group. In acupuncture group and model group, 4-vessel-occlusion method was used to make a rat model of cerebral ischemia reperfusion. Acupuncture group used electroacupuncture on points Quchi and Zusanli, 6 hours after reperfusion. The rats of each group were killed and ELISA was used for the measurement of IL-1β in brain tissues.ResultsIL-1β in brain tissue in model group increased significantly compared with sham operation group and acupuncture group(P<0-001). No significant differences were found between sham operation group and acupuncture group (P>0-05).ConclusionAcupuncture can lower IL-1β content of brain tissue of rats with brain ischemia/reperfusion injury.

Key words: cerebral ischemia-reperfusion, electroacupuncture, interleukin-1β