《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (4): 322-324.

• 论文 • 上一篇    下一篇

二苯乙烯苷对过氧化氢诱导的PC12细胞损伤的保护作用

马海涵1，邵阳2，陈力学3，杨期东2   

  1. 1.解放军第一五三医院神经内科，河南郑州市 450042；2.中南大学湘雅医院神经内科，湖南长沙市 410008；3.重庆医科大学第一附属医院重庆市神经病学重点实验室，重庆市 400016。
  • 收稿日期:2010-02-02 修回日期:2010-03-18 出版日期:2010-04-25 发布日期:2010-04-25
  • 通讯作者: 邵阳

Protective Effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside on PC12 Cells Injury Induced by H2O2

MA Hai-han, SHAO Yang, CHEN Li-xue, et al.   

  1. Department of Neurology, 153 Hospital of PLA, Zhengzhou 450042, China
  • Received:2010-02-02 Revised:2010-03-18 Published:2010-04-25 Online:2010-04-25

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摘要： 目的探讨二苯乙烯苷(TSG)对过氧化氢(H2O2)所致PC12细胞损伤的保护作用。方法培养PC12细胞给予H2O2(100 μmol/L)诱导细胞凋亡，实验分为空白对照组、H2O2损伤组、高剂量组(TSG 120 μg/L+H2O2）、中剂量组(TSG 60 μg/L+H2O2)和低剂量组(TSG 30 μg/L+H2O2)。TSG处理2 h后，加入H2O2损伤细胞，4 h后取细胞测定乳酸脱氢酶(LDH)漏出量，采用硫代巴比妥酸比色法测定丙二醛(MDA)，黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性，噻唑兰(MTT)法检测细胞活力，免疫组织化学方法检测bcl-2的蛋白表达。结果TSG能提高细胞活力，降低LDH漏出和细胞凋亡率(P<0-05)。0-1～1 μmol/L TSG处理后bcl-2的蛋白表达降低(P<0-05)。结论TSG能减少H2O2对PC12细胞的损伤。

关键词: 二苯乙烯苷, H2O2, PC12, 细胞损伤

Abstract: ObjectiveTo study the protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on the PC12 cells injury induced by H2O2.MethodsAll cells were divided into 5 groups: the saline control group(control group), Oxidative damage PC12 cells model group(H2O2 group) which was induced by H2O2, and TSG treated group, which oxidative damage PC12 cells model which was induced by H2O2 after given TSG 2 h at TSG 120 μg/L(TSG 120 μg/L+H2O2 group), 60 μg/L(TSG 60 μg/L+H2O2 group) and 30 μg/L(TSG 30 μg/L+H2O2 group) once. The survival rate of the cells was determined by MTT method, the content of lactate dehydrogenase (LDH) was determined by ultraviolet spectrophometry and the content of malonyldialdehyde (MDA) and superoxide dismutase (SOD) activity was measured respectively by thiobarbituric aicd and xanthine oxidese method. Immunohistochemitry method were used to detect the expression of bcl-2.ResultsTSG reduced obviously cells injury induced by H2O2. 30～120 μg/L TSG improved the cells survival rate, reduced LDH releasing and MDA content, increased SOD activity, and decreased the expression of bcl-2 in the PC12 cells injury induced by H2O2(P<0-05, P<0-01). ConclusionTSG has significantly potective effect on the PC12 cells injury induced by H2O2.

Key words: 4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG), H2O2, PC12, cell injury