《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (3): 205-208.

• 论文 • 上一篇    下一篇

脊髓重建管对T8损伤大鼠后肢运动终板的调节与维持

苏增艳，杨朝阳，李晓光   

  1. 首都医科大学北京神经科学研究所，北京市神经再生修复研究重点实验室，教育部神经变性病学重点实验室，北京市 100069。
  • 收稿日期:2010-01-15 修回日期:2010-03-12 出版日期:2010-03-25 发布日期:2010-03-25
  • 通讯作者: 李晓光

Adjustment and Maintenance of Spinal Cord Reconstruction Tubes on Hindlimb Motor End-plates about Spinal Cord Injury at T8 in Rats

SU Zeng-yan，YANG Zhao-yang，LI Xiao-guang.   

  1. Neuroscience Research Institute of Capital Medical University; Key Laboratory of Beijing Neuroregeneration Repair Research; Key Laboratory of Neural Degeneration of Ministry of Education; Beijing 100069, China
  • Received:2010-01-15 Revised:2010-03-12 Published:2010-03-25 Online:2010-03-25

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摘要： 目的观察大鼠脊髓损伤后及脊髓重建管诱导脊髓再生修复过程中后肢骨骼肌运动终板形态结构与不同类型肌纤维终板区乙酰胆碱酯酶(AChE)的变化情况。方法43只成年Wistar大鼠随机分为脊髓T8单纯损伤组（Cx组）、脊髓T8损伤+脊髓重建管移植修复组（CxTp组）和正常对照组（Co组），术后2周、4周、3个月、6个月、12个月经灌注固定后取出大鼠双下肢腓肠肌、比目鱼肌和趾长伸肌，分别用氯化金染色（压片法）观察运动终板结构形态和Karnovsky-Roots直接法染色检测AChE的变化。结果Cx组和CxTp组大鼠术后运动终板结构形态与AChE活性均发生改变。Cx组终板自术后3个月起发生明显的退行性改变，AChE活性亦呈线性下降；CxTp组终板结构与形态相对稳定，没有退变迹象，术后3个月起AChE 活性显著高于同时间点Cx组。结论大鼠脊髓损伤后，运动终板发生进行性退变，脊髓重建管移植修复可以阻止终板的退行性变，使其向着利于神经康复的方向发生着AChE的复活与结构形态的可塑性变化。

关键词: 脊髓损伤, 脊髓修复, 运动终板, 乙酰胆碱酯酶(AChE), 大鼠

Abstract: ObjectiveTo observe the hindlimb motor end-plate morphology and activity of acetyl cholinesterase (AChE) in different types of muscle fiber end-plate areas after spinal cord injury and spinal cord reconstruction tubes inducing spinal cord regeneration. Methods43 adult Wistar rats were randomly divided into spinal cord transected group at T8 (Cx group), spinal cord transected with transplantation of reconstruction tubes group (CxTp group) and control group (Co group). 2 weeks, 4 weeks, 3 months, 6 months and 12 months after operation, gastrocnemius, soleus and extensor digitorum longus were dissected respectively, and stained with gold chloride to observe the motor end-plate and stained with Karnovsky-Roots to detect AChE. ResultsIn Cx group, the end-plates degenerated since 3 months after operation, while the AChE activity declined. In CxTp group, end-plate structure and morphology were relatively stable and there were no signs of degeneration. ConclusionAfter spinal cord injury, motor end-plates undergo degeneration. The spinal cord reconstruction tubes graft can prevent end-plate degeneration and benefit for AChE reactivation and motor end-plate morphological and structural plasticity towards the direction of neurological rehabilitation.

Key words: spinal cord injury, spinal cord repair, motor end-plate, acetylcholine esterase (AChE), rats