《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (1): 29-31.

• 论文 • 上一篇    下一篇

谷氨酸引起培养大鼠星形胶质细胞水通道蛋白4表达动态变化

路杨，师忠芳，袁芳，韩明   

  1. 首都医科大学北京市神经外科研究所，北京市 100050。
  • 收稿日期:2009-11-09 修回日期:2010-01-14 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 袁芳

Glutamate-induced Dynamic Expression of Aquaporin 4 in Cultured Rat Astrocytes

LU Yang, SHI Zhong-fang, YUAN Fang, et al.   

  1. Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China
  • Received:2009-11-09 Revised:2010-01-14 Published:2010-01-25 Online:2010-01-25

PDF (PC)

被引次数

2

摘要： 目的研究谷氨酸是否引起培养大鼠星形胶质细胞水通道蛋白4(AQP4)表达变化。方法利用传代培养至10 d左右的大鼠星形胶质细胞，给予1 mmol/L L-谷氨酸钠，分别作用1 h、3 h、6 h、12 h、24 h、48 h后进行细胞形态学观察、GFAP免疫化学染色及荧光定量PCR检测AQP4 mRNA表达变化。结果谷氨酸作用1 h后星形胶质细胞出现肿胀并随着作用时间延长肿胀持续，AQP4 mRNA 3 h时开始出现表达降低（P＜0.05），后随谷氨酸作用时间延长先下调后上调，12 h达最低（P<0.01），48 h升至高于正常（P<0.05）。结论AQP4可能在谷氨酸引起星形胶质细胞肿胀发生发展中发挥作用。

关键词: 谷氨酸, 水通道蛋白4, 星形胶质细胞, 大鼠

Abstract: ObjectiveTo determine whether glutamate induces the alteration of aquaporin 4 (AQP4) expression in cultured rat astrocytes. MethodsThe secondary cultured astrocytes were treated with 1 mmol/L L-glutamate for 1 h, 3 h, 6 h, 12 h, 24 h and 48 h. The morphologic changes of astrocytes were observed through microscope after GFAP immunostaining and AQP4 mRNA expression were detected with real-time PCR. ResultsThe astrocytes swelled when exposed to glutamate for 1 h and remained with prolonged treatment. Meanwhile, the AQP4 mRNA expression were early down-regulated and subsequently up-regulated, featured with the lowest AQP4 mRNA level at 12 h after treatment (P<0.01) and higher at 48 h (P<0.05). ConclusionAquaporin 4 may be involved in the occurrence and development of astrocyte swelling induced by glutamate.

Key words: glutamate, aquaporin 4, astrocyte, rat