《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (1): 27-28.

• 论文 • 上一篇    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠神经元损伤的影响

王文1，许栋明2，相婕1，李蕾1，王培昌1，艾厚喜1，张丽1，李林1   

  1. 1.首都医科大学宣武医院药物室，北京市 100053；2.科技部火炬高技术产业开发中心，北京市 100038。
  • 收稿日期:2009-10-14 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Effects of Morroniside on Rats Neurons in Focal Cerebral Ischemia/Reperfusion Injury

WANG Wen, XU Dong-ming, XIANG Jie, et al.   

  1. Xuanwu Hospital of Capital Medical University， Beijing 100053, China
  • Received:2009-10-14 Revised:1900-01-01 Published:2010-01-25 Online:2010-01-25

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被引次数

4

摘要： 目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠皮层超氧化物歧化酶及神经细胞数量的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，再灌注3 d或7 d。以维生素E(VE)为阳性对照药，采用分光光度法检测大鼠皮层超氧化物歧化酶活性，免疫组化法观察神经细胞数量变化。结果莫诺苷（270 mg/kg）能显著增强大鼠超氧化物歧化酶的活性，莫诺苷（30 mg/kg、90 mg/kg、 270 mg/kg）能抑制神经元数量减少。结论莫诺苷能通过增强大鼠皮层抗氧化能力而抑制脑缺血再灌注引起的神经元损伤。

关键词: 莫诺苷, 缺血再灌注, 超氧化物歧化酶, 神经保护

Abstract: ObjectiveTo investigate the effects of morroniside on super oxide dismutase (SOD) and neurons in rats cortex with focal cerebral ischemia/reperfusion. MethodsThe animal model was induced by middle cerebral artery occlusion with suture embolus, cerebral ischemia 30 min and reperfusion 3 d or 7 d. Vitamin E for the positive control. The content of SOD was detected with spectrophotometry and the nerve cells was observed with immunohistochemistry. ResultsCompared with model group, morroniside （270 mg/kg）increased the activity of SOD and the number of neurons （30 mg/kg, 90 mg/kg, 270 mg/kg） significantly. ConclusionMorroniside may have neuroprotective effect and increasing the activity of SOD in rats cortex.

Key words: morroniside, ischemia/reperfusion, super oxide dismutase (SOD), neuroprotective