《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (12): 1122-1124.

• 论文 • 上一篇    下一篇

地黄苏合香合剂对早期肝性脑病大鼠脑内外周型苯二氮受体的影响

刘赫1，刘雁勇1，杨楠1，纪超1，左萍萍1，龚韬2，廖磊2，侯晓明2，蔡哲3   

  1. 1. 中国医学科学院基础医学研究所，北京协和医学院基础学院药理室，北京市 100005；2.北京市中药研究所中药化学室，北京市 100035；3.卫生部中日友好医院临床医学研究所免疫学研究室，北京市 100029。
  • 收稿日期:2011-07-28 修回日期:2011-09-27 出版日期:2011-12-25 发布日期:2011-12-25
  • 通讯作者: 左萍萍

Effects of Rehmannia and Storesin on Peripheral-type Benzodiazepine Receptors in Early Hepatic Encephalopathy Rats

LIU He,LIU Yan-yong, YANG Nan,et al.   

  1. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Beijing 100005, China
  • Received:2011-07-28 Revised:2011-09-27 Published:2011-12-25 Online:2011-12-25

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摘要： 目的观察中药地黄、苏合香(地苏合剂)对早期肝性脑病大鼠脑皮层线粒体外周型苯二氮受体(PBRs)活性的影响。方法采用CCl4致化学性肝损伤大鼠模型，阳性药组给予乳果糖6g/kg，治疗组分别给予不同剂量的地苏合剂，同位素法检测PBRs。结果模型组PBRs结合活性较对照组明显升高(P<0.01)，中、高剂量组PBRs结合活性较模型组降低(P<0.05)；模型组脑皮层线粒体PBRs的最大结合容量(Bmax)值较对照组明显升高(P<0.01)，高剂量组较模型组明显降低(P<0.01)。结论地苏合剂可能通过降低早期肝性脑病大鼠脑皮层线粒体PBRs结合活性，减少神经损伤，从而延缓肝性脑病的发生、发展。

关键词: 地黄, 苏合香, 肝性脑病, 外周型苯二氮受体, 大鼠

Abstract: Objective To assess the effects of Rehmannia and Storesin (RS) on peripheral-type benzodiazepine receptors (PBRs) in early hepatic encephalopathy (HE) rats. Methods CCl4 was used to induce the HE model. The benzodiazepine binding sites of PBRs in rats cortex were studied using the specific ligands [3] PK11195. Lactulose was used in the positive medicine group, and the treatment groups received different dosages of RS. Results The specific binding and the Bmax value of [3] PK11195 both significantly increased in the model group than in the control group (P<0.01). The specific binding decreased in the medium dosage group and the high dosage group than in the model group (P<0.05), and the Bmax value of [3] PK11195RS-H decreased in the high dosage group than in the model group (P<0.01). Conclusion Rehmannia and Storesin is effective on early HE rats by decreasing the specific binding of PBRs, which could reduce the neural injury.

Key words: Rehmannia, Storesin, hepatic encephalopathy, peripheral-type benzodiazepine receptors, rats