《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (9): 844-847.

• 论文 • 上一篇    下一篇

电刺激对大鼠脊髓损伤后神经胶质纤维酸性蛋白与白细胞介素-1α表达的影响

张莹莹1a，李俊岑1a，饶莹1a，杨拯1b，张晓1b，郑蕖1c，许丽丽1c   

  1. 1.成都医学院，a: 临床医学系；b: 实验技术教研室；c: 基础医学院，四川成都市 610081。
  • 收稿日期:2011-04-14 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 张晓

Effects of Electrical Stimulation on the Expression of Glial Fibrillary Acidic Protein and Interleukin-1 Alpha in Adult Rats with Spinal Cord Injury

ZHANG Ying-ying, LI Jun-cen , RAO Ying, et al.   

  1. Chengdu Medical College, Chengdu 610083, Sichuan, China
  • Received:2011-04-14 Revised:1900-01-01 Published:2011-09-25 Online:2011-09-25

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被引次数

26

摘要： 目的探讨电刺激对脊髓损伤后神经胶质纤维酸性蛋白(GFAP)、白细胞介素-1α(IL-1α)表达的影响。方法健康成年SD大鼠72 只随机分为正常组、损伤组、电刺激组。采用Allen's 法将后两组复制为脊髓T9损伤模型。术后对电刺激组大鼠进行电刺激治疗7 d。3 组均进行BBB评分，免疫组织化学检测GFAP 与IL-1α的表达情况。结果损伤组和电刺激组BBB评分均小于正常组(P<0.05)，伤后5 d、7 d，电刺激组较损伤组BBB 评分增加(P<0.05)。损伤组、电刺激组GFAP 阳性表达均在伤后5 d 达到高峰，伤后5 d、7 d，电刺激组GFAP 表达低于损伤组(P<0.05)；IL-1α阳性表达在伤后7 d 内持续上升，伤后5 d、7 d，电刺激组IL-1α表达低于损伤组(P<0.05)。结论电刺激能抑制GFAP 与IL-1α的表达，可能有利于减轻炎症反应，减少胶质瘢痕形成。

关键词: 脊髓损伤, 电刺激, 神经胶质纤维酸性蛋白, 白细胞介素-1α

Abstract: Objective To investigate the effects of electrical stimulation on the expression of glial fibrillary acidic protein (GFAP) andinterleukin-1 alpha (IL-1α) in adult rats with spinal cord injury. Methods 72 adult SD rats were randomly divided into damage group (n=24), electrical stimulation group (n=24) and normal group (n=24). The spinal cord incomplete injury model on T9 was made with Allen'smethod in the former 2 groups. The rats in electrical stimulation group accepted electrical stimulation for 7 d. All the rats were evaluatedwith the Basso, Beattie & Bresnahan locomotor rating scale (BBB scale), and the expression of GFAP and IL-1α were determined with immunohistochemistry.Results The BBB scores in both the damage group and electrical stimulation group were significantly less than that inthe normal group (P<0.05), and it was more in the electrical stimulation group than in the damage group 5 and 7 d after injury. The expressionsof the GFAP significantly increased after injury to the peak on 5th day, while it was less in the electrical stimulation group than in thedamage group 5 and 7 d after injury (P<0.05). The expressions of the IL-1α increased continually after injury, while it was less in the electricalstimulation group than in the damage group 5 and 7 d after injury (P<0.05). Conclusion Electrical stimulation can inhibit the expressionof GFAP and IL-1α, that reduce inflammation and glial scar formation.

Key words: spinal cord injury, electrical stimulation, glial fibrillary acidic protein, interleukin-1 alpha